Eurasian Journal of Biosciences

The effect of Annona muricata and Urtica dioica on killing the proscolex of Echinococcus granulosus


This study showed the effect of Annona muricata and Urtica dioica on killing the proscolex of Echinococcus granulosus parasite. The echinococcus cysts was isolated from the livers of slaughtered sheep in Mosul city. The vitality of the cysts was studied under the microscope using a 0.1% aqueous aucine. concentrations were used (10, 20, 30, 40, 50, 60, 70, 80, 90) µg/ml from an extract of Annona muricata and Urtica dioica plants to study its influence on the vitality number of the proscolex. These proscolex were treated with the extract for different time periods ranging from (0, 15, 30, 45, 60) minutes, the study showed a significant effect of Urtica dioica plant on killing the proscolex of Echinococcus granulosus. Flavonoids had a fatal effect on killing the proscolex in the concentrations (10, 20, 30, 40, 50, 60, 70, 80, 90) µg/ml for time periods ranging from (0, 15, 30, 45, 60) minutes. The 90 µg/ml showed a high effect on killing the proscolex. Alkaloids also showed a similar effect to flavonoids on killing the proscolex in the same concentrations and time periods, while the alkaloids were extracted from Annona muricata has a moderately effective on killing the proscolex in the same concentrations and time periods. While the cleucosides and flavonoids extracted from Urtica dioica did not show any effect in the same concentrations and time periods.


  • Al-Khafaji, N.J., Al– Zubaedi , R.M., ( 2013 ) . The Clinical and Hematological changes in rabbits exposed to leaves of Urtica dioica under experimental conditions . Kufa Journal For Veterinary Medical Sciences Vol. (4) No. (2).
  • Banerjee , A., Das, D., Maji B.K., Mukherjee, S.( 2018 ) .Anticancer effects of Annona muricata with its acetogenins as bioactive compound. Cytology & Histology International Journal . 2 : (1).
  • Cardozo , C. J.M., Lozano, V. V., Betancur,D.P .Y.Velásquez, H .J.C., Valenzuela, J.R.K.(2012). Physiological and Physico-Chemical Characterization of the Soursop Fruit (Annona muricata L. cv. Elita) Rev.Fac.Nal.Agr.Medellín 65(1):-6477-6486.
  • Fatemeh, E ., Mohammad , A . M., Farzaneh , M., Mohsen , G., Seyed , H. H ., (2018 ) . Molecular Characteristics of Echinococcus granulosus Strains Isolated from Iranian Camel Using High Resolution Melting Analysis of atp6 and cox1 Genes. Avicenna J Clin Microbiol Infect, Volume5, Issue2.
  • Gemmell M.A., & Lawson J.R. (2016).” Epidemiology and control of hydatid disease In: The biology of Echinococcus and hydatid disease “.Thompson R.C.A. (ed.,) Allen and Dun Win. London . Avicenna J Clin Microbiol Infect, Volume 5, Issue 2.
  • Gholami, S ., Tanzifi , A ., Sharif, M ., Daryani, A ., Rahimi, M ., Mirshafiee, S . Sarvi, S. (2018 ). Demographic aspects of human hydatidosis in Iranian general Veterinary World, 11(10): 1385-1396.
  • Marquardt W.C., Demarec R.S., & Grieve R.B., (2012). “Parasitology and vector biology”. 2nd. ed., Harcourt Academic Press Toronto, 335-339.
  • Moghadamtousi, S. Z., Fadaeinasab, M . Nikzad, S ., Gokula Mohan ,G ., Ali , H . M., Kadir , H . A. ( 2015 ) . Annona muricata (Annonaceae): A Review of Its Traditional Uses, Isolated Acetogenins and Biological Activities. Int. J. Mol. Sci., 16, 15625-15658.
  • Moro,P . L ., McDonald,J ., Gilman, R.H. Silva, B. Verastegui, M. Malqui, V .Lescano, G. Falcon, N. Montes, G. Bazalar, H. (2017). Epidemiology of Echinococcus granulosus infection in the central Peruvian Andes. Bulletin of the Wor Health Organization, 75 (6): 553-561.
  • Odok, G. N., Unaeze, H. C., Ogueri, E. I., Essien, U. A., Ukpong, C. A., Bassey, J. E., ... & Ohajianya, D. O. (2018). Climate Variability, Incidences and Mitigation Strategies among Poultry Farmers in Abia State, Nigeria. International Journal of Sustainable Agricultural Research, 5(3), 38-45.
  • Patel, S. Patel , K.J .(2016) . A review on a miracle fruits of Annona muricata .Journal of Pharmacognosy and Phytochemistry. 5(1): 137-148.
  • Pedro, M., Peter M.S. (2019) . Echinococcosis: a review . International Journal of Infectious Diseases.13: 125—133.
  • Rausch, R.L. (2017).” Echinocococcus granulosus : Biology and Ecology In: Compendium on cystic Echinococcosis in Africa and in Middle Eastren Countries with special reference to Morocco”. Andersen, F.L., H. Ouhelli and M. Kachani (Editors) Brigham Young Univer Print Service Provo, 4T84602, USA, PP 18 -54.
  • Salih, E.Dh., Arif D. J. A. (2014 ) . Antibacterial effect of nettle (Urtica dioica) . AL- Qadisiya Journal of Vet. Med. Sci. Vol. 13 No. 1.
  • Smith C.J., Hussain J.I. & Allen J.C. (2014).” Inhibition of cell proliferation by polyamines does not depend on the cytotoxicity of acroline”. Biochem. Soc. Trans., 13: 326.
  • Smyth J.D. & Barett N.G. (2017). “Procedure for testing the viability of human hydatid cysts following surgical removal especially after chemotherapy”. Trans. Roy. Soc. Trop. Med. Hyg., 14: 649-652
  • Venkastesan P.(2018). Albendazole .J. Antimicrobial. Chemotherap. 41: 145-147


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