Eurasian Journal of Biosciences

Molecular study of Rota A viruses at many farm animals in Iraq
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 3051-3057
  • Published Online: 17 Sep 2020
  • Open Access Full Text (PDF)


Background: The species A of Rotavirus is considered as of medical and veterinary important pathogen, worldwide distributed and responsible for acute gastroenteritis particularly at juvenile ages, the viruses explain marked genetic diversity which represented by numerous number genotypes infect different vertebrate hosts. 
Methods: About 942 fecal samples had been taken directly from the rectum of 403 cattle, 236 sheep,174 goats and 129 of camels, their age younger than 6 months and suffering from diarrhea at different Iraqi Governorates; advanced specific molecular technique was implemented, Reverse Transcription -modern- Real Time PCR technique (rRT- PCR) with utilizing of a new generation thermo-cycler to obtain reliable result regarding exact viral genotypes in these animals at Iraq. 
Results: The RVAs were observed in 29.61% of diarrheic animals the percentages of infection among these animals as followings: cattle, sheep, goats and camels were 31.51%, 29.23%, 27.58% and 27.13%, respectively, the distribution pattern of viral genotypes as following: in cattle: G6, G8, G10, P[1] and P[5] in addition to P[11]; in sheep: G3, G6, G10, P[1] and P[11]; in goats: G6 and also P[5]; in camels: G10, P[1] and P[11]. G10 was the most dominant among specific genotypes in all animals in this study.
Conclusions: According to the author’s knowledge, the present study is the first study that recorded G3, G6, G8, G10, and also P [1], P [5] besides to P [11] in above animals at Iraq, which offers a valuable and reliable epidemiological information pertaining RAVs genotypes that were urgently needed for both human and animal necessary hygiene regards.


  • Abo Hatab, E. M., Hussein, H. A., El-Sabagh, I. M., Saber, M. S. Isolation and antigenic and molecular characterization of G10 of group A rotavirus in camel. Int J Virol, 2009; 5:18-27.
  • Al-Ruwaili, M. A., Khalil, O.M., Selim, S. A. Viral and bacterial infections associated with camel (Camelus dromedarius) calf diarrhea in North Province, Saudi Arabia. Saudi J Biol Sci, 2012; 19(1):35-41.
  • Arias, C. F., Silva-Ayala, D., López, S. Rotavirus Entry: a Deep Journey into the Cell with Several Exits. J Virol, 2015; 89(2): 890-93.
  • Badur, S., Öztürk, S., Pereira, P., AbdelGhany, M., Khalaf, M., Lagoubi, Y., Ozudogru, O., Hanif, K., Saha, D. Systematic review of the rotavirus infection burden in the WHO-EMRO region. Hum Vaccin Immun-other, 2019; 15(11):2754-68.
  • Barua, S., Rakib, M. T., Rahman, M. M., Selleck, S., Masuduzzaman, M., Siddiki, A., Hossain, M. A., Chowdhury, S. Disease burden and associated factors of rotavirus infection in calves in south-eastern part of Bangladesh. Asi J Med Biol Res, 2019; 5: 107-16.
  • Boehm, A. B., Silverman, A. I., Schriewer, A., Goodwin, K. Systematic review and meta-analysis of decay rates of waterborne mammalian viruses and coliphages in surface waters. Water Res, 2019; 164(1):11489-98.
  • Chakraborty, P., Bhattacharjee, M. J., Sharma, I., Pandey, P., Barman, N. N. Unusual rotavirus genotypes in humans and animals with acute diarrhoea in Northeast India. Epidemiol. Infect, 2016; 144: 2780-89.
  • Changotra, H., Vij, A. Rotavirus virus-like particles (RV-VLPs) vaccines: An update, 2017; 27(6):195-105.
  • Clark, A. D., Hasso-Agopsowicz, M., Kraus, M. W., Stockdale, L. K., Sanderson, C. F. B., Parashar, U. D., Tate, J. E. Update on the global epidemiology of intussusception: a systematic review of incidence rates, age distributions and case-fatality ratios among children aged <5 years, before the introduction of rotavirus vaccination. Int J Epidemiol, 2019;48 (4):1316-26.
  • Dash, S., Tewari, A., Kumar, K., Goel, A. and Bhatia, A. Detection of Rotavirus from diarrhoeic cow calves in Mathura, India. Vet. World, 2011; 4(12):554-56.
  • Desselberger, U. Review: Rotaviruses. Virus Res, 2014; 190:75-96.
  • Fujii1, Y., Doan, Y. H., Wahyuni, R. M., Lusida, M. I., Utsumi, T., Shoji, I., Katayama, K. Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set. Front Micriobiol, 2019; 10:647-53.
  • Gazal, S., Taku, A. K., Kumar, B. Predominance of rotavirus genotype G6P[11] in diarrhoeic lambs. Vet J, 2012; 193(1):299-300.
  • Ghosh, S., Kobayashi, N. Exotic rotaviruses in animals and rotaviruses in exotic animals. Virus Dis, 2014; 25(2):158-72.
  • Gouvea, V., Glass, R. I., Woods, P., Taniguchi, K., Clark, H. F., Forrester, B., Fang, Z. Y. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol, 1990; 28:276-82.
  • Hasso-Agopsowicz, M., Ladva C. N., Lopman, B., Sanderson, C., Cohen, A. L., Tate, J. E., Riveros, X., Henao-Restrepo, A. M., Clark, A. Global Review of the Age Distribution of Rotavirus Disease in Children Aged <5 Years Before the Introduction of Rotavirus Vaccination. Clin Infect Dis, 2019; 69(6):1071-78.
  • Heide, R., Koopmans, M. P. G., Shekary, N., Houwers, D. J., van Duynhoven, Y. T. H. P., van der Poe, W. H. M. Molecular Characterizations of Human and Animal Group A Rotaviruses in The Netherlands. Jour Clin Microbiol, 2005; 43(2):669-75.
  • Hossain, M. B., Rahman, S., Watson, O. J., Islam, A., Rahman, S., Hasan, R., Kafi, M. A., Osmani, M. G., Epstein, J. H., Daszak, P., Haider, N. Epidemiology and genotypes of group A rotaviruses in cattle and goats of Bangladesh, 2009-2010. Infec Gene Evol, 2020; 20(1):1348-67.
  • Jere, K. C., Esona, M. D., Ali, Y. H., Peenze, I., Roy, S., Bowen, M. D., Saeed, I. K., Khalafalla, A. M. I., Nyaga, M. M., Mphahlele, J. M., Steele, D. A., Seheri, M. L. Novel NSP1 genotype characterized in an African camel G8P[11] rotavirus strain. Infect Genet Evol, 2014; 21:58-66.
  • Jindal, S. R., Maiti, N. K., Oberoi, M. S. Genomic diversity and prevalence of Rotavirus in cow and buffalo calves in northern India. Rev Sci Tech, 2000; 19(3):871-76.
  • Jothikumar, N., Kang, G., Hill, V. R. Broadly reactive TaqMan assay for real-time RT-PCR detection of rotavirus in clinical and environmental samples. J Virol Methods, 2009; 155(2):126-31.
  • Liu, J., Lurain, K., Sobuz, S.U., Begum, S., Kumburu, H., Gratz, J., Kibiki, G., Toney, D. Molecular genotyping and quantitation assay for rotavirus surveillance. J Virol Meth, 2015; 213:157-63.
  • Lu, H. L., Ding, Y., Goyal, H., Xu, H. G. Association Between Rotavirus Vaccination and Risk of Intussusception Among Neonates and Infants: A Systematic Review and Meta-analysis. JAMA, 2019; 2(10):12458-68.
  • Luchs, A., Carmo, M., Timenetsky, S. T. Group A rotavirus gastroenteritis: post-vaccine era, genotypes and zoonotic transmission. Rev Bas Sci, 2016; 14(2):278-87.
  • Medeiros, T. S., Lorenzetti, E., Alfierib, A. F. and Alfier, A. A. (2019). G and P genotype profiles of rotavirus A field strains circulating in beef and dairy cattle herds in Brazil, 2006–2015. Comp Immun Microb Infec Dis. 64:90-98.
  • Mirza, A. H., Das, S., Pingle, M. R., Rundell, M. S., Armah, G., Gyan, B., Hodinka, R. L., Larone, D. H., Spitzer, E. D., Barany, F., Golightly, L. M. A Multiplex PCR/LDR Assay for Viral Agents of Diarrhea with the Capacity to Genotype Rotavirus. Sci Repo, 2018; 8:13215-26.
  • Pérez-Luna, Y. C., Sánchez-Roque, Y., & Berrones Hernández, R. (2018). Physicochemical Characterization of Compost Mixtures Enriched with Agroindustrial Waste. Canadian Journal of Agriculture and Crops, 3(1), 33-41.
  • Rodríguez, J. M., Luque, D. Review: Structural Insights into Rotavirus Entry. Adv Exp Med Biol, 2010; 1215:45-68.
  • Suzuki, H. Rotavirus Replication: Gaps of Knowledge on Virus Entry and Morphogenesis. J Exp Med, 2019; 248(4):285-96.
  • Tamim, S., Matthijnssens, J., Heylen, E., Zeller, M., Ranst, M. V., Salman, M., Hasan, F. Evidence of zoonotic transmission of VP6 and NSP4 genes into human species A rotaviruses isolated in Pakistan in 2010. Arch Virol, 2019; 164:1781-91.
  • Taniguchi, K., Komoto, S. Genetics and reverse genetics of rotavirus. Curr Open Virol, 2012; 2:399-407


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