Eurasian Journal of Biosciences

Investigating the association of vitamin D levels with RF and HMGB1 in Rheumatoid arthritis patients in Basra, Iraq


Background: Rheumatoid arthritis (RA) is autoimmune disease characterized by swollen joints and the presence of autoantibodies. Many factors can affect the susceptibility of a person to this autoimmune disease, one of these factors is the level of vitamin D (VD3). The aim of the study is investigating the relationships of VD3, RF and HMGB1 in RA patients in Basra. Materials and Methods: A total of 154 participants were included RA patients (n=113) and healthy control (HC) (n=41) from both sexes, age range 23-75 years. The sera of RA patients and HC were tested for the level of Rheumatoid Factors (RF) included (IgG and IgM), VD3 and Human HMGB1 protein measurement by ELISA. Results: Our results showed that the level of RF were significantly higher and VD3 was significantly lower in RA patients in compare with HC. Furthermore, serum HMGB1 levels were higher in RA patients without treatment, but decreases upon therapy in patients while it was low in HC. A significant differences noticed in serum HMGB1 levels in RA patients depended on the types of drugs. Positive correlation between VD3, HMGB1 and the disease progress, while negative correlations were found between VD3, RF and HMGB1. Conclusion: The status of VD3 in RA patients living in Basra and its relation to the levels of RF and the pro inflammatory mediator HMGB1, documenting that VD3 deficiency play a role in causing the inflammation during RA.


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