Eurasian Journal of Biosciences

Growth of Streptococcus mutans in developer solutions with water supply and aquadest solvents
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 3809-3813
  • Published Online: 30 Sep 2020
  • Open Access Full Text (PDF)


Background: Radiographic processing involves intraoral film which contaminated with oral cavity that has Streptococcus mutans in it, then it can contaminate equipment in a dark room. Therefore, it can be the source to cross-infection. Developer solution made at Universitas Airlangga Radiology Clinic uses tap water from river in Surabaya as its solvent. The results of the tap water quality test shows various minerals such as manganese, zinc, and iron. Manganese, iron, and zinc are proven can influence the growth of Streptococcus mutans and biofilm development. This bacteria can contaminate the developer solution during the radiographic process and its growth can be affected by the mineral in the tap water as a developer solvent.
Purpose: To determine the growth of Streptococcus mutans in developer solution with water supply and aquadest as solvent and to know the differences between them.
Method: The concentrated developer was dissolved in the tap water and aquadest then routinely used at radiology clinic in dental hospital of Universitas Airlangga. Samples was taken before any films washed as control and sample of test taken 16 times each. Every sample can be taken after 8 films were washed. Control and test samples were diluted on BHIB media, planted in TYC media, and incubated for 2x24 hours. Bacteria were identified and then the number of colonies can be counted.
Result: There was growth of Streptococcus mutans as much as one colony in two samples of developer solution with a water supply solvent. There was no growth of Streptococcus mutans bacteria in the developer solution with aquadest solvent. There was no significant difference in growth of Streptococcus mutans bacteria in the solution of the developer of the water supply and the aquadest solvent.
Conclusion: The growth of Streptococcus mutans bacteria in the developer solution with a water supply solvent is more increasing than the aquadest solvent.


  • Ahn, S. J., Ahn, S. J., Wen, Z. T., Brady, L. J., & Burne, R. A. (2008). Characteristics of biofilm formation by Streptococcus mutans in the presence of saliva. Infection and immunity, 76(9), 4259-4268.
  • Aranha, H., Strachan, R. C., Arceneaux, J. E., & Byers, B. R. (1982). Effect of trace metals on growth of Streptococcus mutans in a teflon chemostat. Infection and Immunity, 35(2), 456-460.
  • Arirachakaran, P., Luengpailin, S., Banas, J. A., Mazurkiewicz, J. E., & Benjavongkulchai, E. (2007). Effects of manganese on Streptococcus mutans planktonic and biofilm growth. Caries research, 41(6), 497-502.
  • Bachman, C. E., White, J. M., Goodis, H. E., & Rosenquist, J. W. (1990). Bacterial adherence and contamination during radiographic processing. Oral surgery, oral medicine, oral pathology, 70(5), 669-673.
  • Fernandes, L. M. P. D. S. R., Zapata, R. O., Rubira-Bullen, I. R. F., & CAPELOZZA, A. L. Á. (2013). Microbiologic cross-contamination and infection control in intraoral conventional and digital radiology. RGO. Revista Gaúcha de Odontologia (Online), 61(4), 609-614.
  • Francesca, B., Ajello, M., Bosso, P., Morea, C., Andrea, P., Giovanni, A., & Piera, V. (2004). Both lactoferrin and iron influence aggregation and biofilm formation in Streptococcus mutans. Biometals, 17(3), 271-278.
  • Göstemeyer, G., Kohls, A., Paris, S., & Schwendicke, F. (2018). Root caries prevention via sodium fluoride, chlorhexidine and silver diamine fluoride in vitro. Odontology, 106(3), 274-281.
  • Indonesia, K. S. (2017). Monosex barb (Osteochilus hasselti) Culture with reduction feed on economic efficiency and cost reduction at net cage in Cirata Reservoir. Current Research in Agricultural Sciences, 4(1), 7-13.
  • Ishaq, W. (2015). Tingkat Penggunaan Radiografi Periapikal pada Dokter Gigi Praktek di Kabupaten Maros Terhadap Perawatan Endodotik. Makassar: Universitas Hasanuddin.
  • Jeani, N., & Andina, M. (2019, July). Wild honey 50% inhibits growth of streptococcus viridans in vitro. In Journal of Physics: Conference Series (Vol. 1246, No. 1, p. 012020). IOP Publishing.
  • Khalil, H. (2013). Microbial contamination of radiographic films in maxillofacial surgery clinics. African Journal of Microbiology Research, 7(37), 4625-4628.
  • Priyono, T. S. C., Yuliani, E., & Sayekti, R. W. (2013). Studi Penentuan Status Mutu Air Di Sungai Surabaya Untuk Keperluan Bahan Baku Air Minum. Jurnal Teknik Pengairan, 4(1), 53-60.
  • Rukayadi, Y., Kim, K. H., & Hwang, J. K. (2008). In vitro anti‐biofilm activity of macelignan isolated from Myristica fragrans Houtt. against oral primary colonizer bacteria. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 22(3), 308-312.
  • SANTOS, R.M.D., Santos, F.L.M.D., Ramacciato, J.C. & Junqueira, J.L.C., (2012). Evaluation of antimicrobial contamination and resistance to Staphylococcus aureus collected from radiographic materials used in dentistry. RGO. Revista Gaúcha de Odontologia (Online), 60(4), pp.467-477.
  • Zhou, X., & Li, Y. (Eds.). (2015). Atlas of oral microbiology: From healthy microflora to disease. Academic press.


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