Eurasian Journal of Biosciences

Genetic detection of in vA, sipB, SopB and sseC genes in Salmonella spp isolated from diarrheic children patients
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 3085-3091
  • Published Online: 17 Sep 2020
  • Open Access Full Text (PDF)


Salmonella Gram-negative bacteria infect human and animals and cause gastroenteritis and typhoid fever. Type III secretion system are important virulence factor of salmonella it basically and functionally associated with flagella meeting systems and typically comprise more than 20 proteins subunits that are found in the internal and external membrane of the bacterial cell. The current study aims to conduct an detection the candidates genes of type III secretion system as important virulence factor of a clinical Salmonella spp. The Patient and methods: current study includes 120 stool samples collected from 120 diarrheic children, age range from (2 to 3 years) for the period from November (2018) to December (2018) from Babylon province Iraq. After culturing samples, Salmonella spp.diagnosed. DNA extraction and PCR were achieved for detection type III secretion system genes of Salmonella spp. The results: out of 120 stool sample, 58 samples were positive for Salmonella spp. we revealed that Salmonella typhi, Salmonella typhimurium, Salmonella arizonae, and Salmonella paratyphi, the most common serovar of Salmonella enterica, was Salmonella typhi at 29.3%. Genetic detection of type III secretion system by PCR technique explains that Inva A was found about at all isolates except one isolate at percentage (93.1%) and SipB occurrence was (18.9%) finally SseC occurrence was (1.7%) whereas SopB was not detected in all salmonella isolates. inva A gene is most reliable gene in the diagnosis of Salmonella spp. and not all isolates contain a different genes of type III secretion system.


  • Abd Elseed, Y. H. A. E. (2015). Comparison between the Widal test and culturing technique in the diagnosis of enteric fever in Khartoum State, Sudan. African Journal of Bacteriology Research, 7(5), 56-59.
  • Boonyom, R., Karavolos, M. H., Bulmer, D. M., & Khan, C. M. A. (2010). Salmonella pathogenicity island 1 (SPI-1) type III secretion of SopD involves N-and C-terminal signals and direct binding to the InvC ATPase. Microbiology, 156(6), 1805-1814.
  • Di Bella, S., Capone, A., Bordi, E., Johnson, E., Musso, M., Topino, S.,... & Petrosillo, N. (2011). Salmonella enterica ssp. arizonae infection in a 43-year-old Italian man with hypoglobulinemia: a case report and review of the literature. Journal of Medical Case Reports, 5(1), 1-4.
  • Diamond, C. E., Leong, K. W. K., Vacca, M., Rivers-Auty, J., Brough, D., & Mortellaro, A. (2017). Salmonella typhimurium-induced IL-1 release from primary human monocytes requires NLRP3 and can occur in the absence of pyroptosis. Scientific Reports, 7(1), 1-9.
  • Espinoza, R. A., Silva Valenzuela, C. A., Amaya, F. A., Urrutia, Í. M., Contreras, I., & Santiviago, C. A. (2017). Differential roles for pathogenicity islands SPI-13 and SPI-8 in the interaction of Salmonella Enteritidis and Salmonella Typhi with murine and human macrophages. Biological research, 50.
  • Gerlach, R. G., & Hensel, M. (2007). Salmonella pathogenicity islands in host specificity, host pathogen-interactions and antibiotics resistance of Salmonella enterica. Berliner und Munchener tierarztliche Wochenschrift, 120(7/8), 317.
  • Kagirita, A. A., Baguma, A., Owalla, T. J., Bazira, J., & Majalija, S. (2017). Molecular characterization of Salmonella from human and animal origins in Uganda. International Journal of Bacteriology, 2017.
  • Kim, J. E., & ju Lee, Y. (2017). Molecular characterization of antimicrobial resistant non-typhoidal Salmonella from poultry industries in Korea. Irish veterinary journal, 70(1), 20.
  • Li, S., Zhang, Z., Pace, L., Lillehoj, H., & Zhang, S. (2009). Functions exerted by the virulence-associated type-three secretion systems during Salmonella enterica serovar Enteritidis invasion into and survival within chicken oviduct epithelial cells and macrophages. Avian Pathology, 38(2), 97-106.
  • Mahajan, R. K., Khan, S. A., Chandel, D. S., Kumar, N., Hans, C., & Chaudhry, R. (2003). Fatal case of Salmonella enterica subsp. arizonae gastroenteritis in an infant with microcephaly. Journal of clinical microbiology, 41(12), 5830-5832.
  • Maskey, A. P., Day, J. N., Tuan, P. Q., Thwaites, G. E., Campbell, J. I., Zimmerman, M.,... & Basnyat, B. (2006). Salmonella enterica serovar Paratyphi A and S. enterica serovar Typhi cause indistinguishable clinical syndromes in Kathmandu, Nepal. Clinical infectious diseases, 42(9), 1247-1253.
  • Mezal, E. H., Stefanova, R., & Khan, A. A. (2013). Isolation and molecular characterization of Salmonella enterica serovar Javiana from food, environmental and clinical samples. International journal of food microbiology, 164(1), 113-118.
  • Rahman, H. (2006). Prevalence & phenotypic expression of sopB gene among clinical isolates of Salmonella enterica. Indian Journal of Medical Research, 123(1), 83.
  • Ramya, P., Madhavarao, T., & Rao, L. V. (2012). Study on the incidence of Salmonella enteritidis in poultry and meat samples by cultural and PCR methods. Vet World, 5(9), 541-545.
  • Sabir, F. K., & Sabir, A. (2018). Effects of Different Storage Conditions on Rooting and Shooting Performance of Grapevine (Vitis Vinifera L.) Cuttings in Hydroponic Culture System. International Journal of Sustainable Agricultural Research, 5(3), 46-53.
  • Soto, S. M., Rodríguez, I., Rodicio, M. R., Vila, J., & Mendoza, M. C. (2006). Detection of virulence determinants in clinical strains of Salmonella enterica serovar Enteritidis and mapping on macrorestriction profiles. Journal of medical microbiology, 55(4), 365-373.
  • Won, G., & Lee, J. H. (2017). Salmonella Typhimurium, the major causative agent of foodborne illness inactivated by a phage lysis system provides effective protection against lethal challenge by induction of robust cell-mediated immune responses and activation of dendritic cells. Veterinary research, 48(1), 66.


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