Eurasian Journal of Biosciences


Background: A mucosal oral ulceration is epithelium damage that occurs anywhere inside the oral cavity. It is deep breakdown in the continuity of oral epithelium occur due to molecular necrosis associated with pain, redness and tenderness of affected site Curcumin was a turmeric extract, its multifaceted biological effects such as, anti-inflammatory, anti-oxidant, anti-carcinogenic and anti-infectious effects. The aim of the present study is to evaluate the effect of topical application of curcumin on mucosal oral ulcer healing.
Materials and methods: Thirty-six male white New Zealand rabbits age between (2-8) months with body weight between (1000-1400 gm), were subjected to traumatic ulcer by ‘punch biopsy’ on the right side of the buccal mucosa, with diameter of (8 mm) and (2mm) depth. The animals divided into two groups; control group: the ulcer treated with sterilized distal water, the experimental group: the ulcer treated with 1% curcumin oral gel. The rabbits were sacrificed at 3rd,7th and 14th days. Clinical assessment of ulcer size and histological and histomorphometric analysis of H&E slide in both control and study groups.
Results: The present study showed that the curcumin treatment reduce mucosal ulcer area from the 3rd day till 7th day and improved ulcer healing at 14th day by activation of epithelial cell, inhibit inflammatory cells, enhance endothelial cells and promote fibroblast cells. While control group showed limited healing process of mucosal ulceration from early period of 3rd day with minimal contraction at the 7th day and incomplete healing at 14th day.


  • ABED S S, (2018) Immunohistochemical Expression of Decorin, and Epidermal Growth Factor Receptor in Induced Traumatic Oral Mucosal Ulcer Treated with Leptin in Rats. Doctoral thesis. Baghdad University, College of Dentistry, Baghdad.
  • Accorint M L, Holland R, Reis A, Bortoluzzi M, (2008) Evaluation of Mineral Trioxide Aggregate and Calcium Hydroxide Cement as Pulp-capping Agents in Human Teeth. JOE.34:89-95.
  • Alam A (2014). Redox signaling regulates commensal-mediated mucosal homeostasis and restitution and requires formyl peptide receptor 1. Mucosal Immunology. 7, 645–655.
  • Broughton G, Janis J E, Attinger C E, (2006). The Basic Science of Wound Healing. Plastic & Reconstructive Surgery, 117, 12S-34S.
  • Chen Z, Hu Y, Li J, Zhang C, Gao F, Ma X, Zhangj, Fu C, Geng F (2019). A feasible biocompatible hydrogel film embedding Periplaneta americana extract for acute wound healing, International Journal of Pharmaceutics S0378-5173(19)30752-5.
  • Cheppudira B, Fowler M, Mcghee L, et al. (2013). Curcumin: a novel therapeutic for burn pain andWound healing. Expert Opin Invest Drugs; 22:1295.
  • Dai M, Zheng X, Xu X, Kong X, Li X, Guo G, et al. (2009). Chitosan- Alginate Sponge: Preparation and Application in Curcumin Delivery for Dermal Wound Healing in Rat. J Biomed Biotechnol, 11:1-8.
  • Donald Plump C, Pharm D (2008) Plumb`s Veterinary Drug Handbook. Sixth edition, Blackwell publishing; 515.
  • Eming S A, Martin P, Tomic-Canic M (2014).Wound repair and regeneration: mechanisms, signaling, and translation. Sci.Transl. Med. 6, 265.
  • Franco E S, Aquino C M F, Medeiros P L et al.,(2012) Effect of a semisolid formulation of Linum usitatissimum L., (Linseed) oil on the repair of skin wounds,” Evidence-Based Complementary and AlternativeMedicine, vol. 2012, Article ID270752, p. 7.
  • Gardekeg J, Nancyn A N, Wayane A B (2004). Curcumin blocks multiple sites of the TGF-b signaling cascade in renal cells. Kidney Int. 66: 112–120
  • Gnepp D R (2009). Diagnostic Surgical Pathology of the Head and Neck. 2nd ed. Philadelphia, PA: Elsevier.
  • Jagetia G C, Rajanikant G K, (2012) Acceleration of wound repair by curcumin in the excision wound of mice exposed to different doses of fractionated γ radiation. INT Wound J. 9(1):76–92.
  • Janis J E, Harrison B, (2016). Wound Healing: Part I. Basic Science. Plast. Reconstr. Surg. Sep;138(3 Suppl):9S-17S.
  • Kant V, Gopal A, Kumar D, Pathak N N., Ram M, Jangir B L, Tandan S K, Kumar D, (2014) Curcumin-induced angiogenesis hastens wound healing in diabetic rats. J. Surg. Res.193:978–988.
  • Kulac M, Aktas C, Tulubas F, et al. (2013). The effects of topical treatment with curcumin on burn wound healing in rats. J Mol Histol. 44: 83.
  • Mallet C, Vittet D, Feige J J, Bailly S, (2006). TGFbeta1 induces vasculogenesis and inhibits angiogenic sprouting in an embryonic stem cell differentiation model: respective contribution of ALK1 and ALK5. Stem Cells.24:2420.
  • Mani H, Sidhu G, Kumari R, Gaddipati J, Seth P, Maheshwari R, (2002). Curcumin differentially regulates TGF-beta1, its receptors and nitric oxide synthase during impaired wound healing. Biofactors, 16:29–43.
  • Margina D, Gradinaru D, Manda G, Neagoe I, And Ilie M, (2013) Membrane effects exerted in vitro by polyphenols—quercetin, epigallocatechin gallate and curcumin—on HUVEC and Jurkat cells, relevant for diabetes mellitus,” Food and Chemical Toxicology.
  • Mayo Clinic. (2020.) Hydrocortisone (Topical Application Route): Proper Use. Retrieved from
  • Meng B, Li J, Cao H, (2013). Antioxidant and Antiinflammatory Activities of Curcumin on Diabetes Mellitus and its Complications. Current pharmaceutical design. 19(11):2101–13.
  • Merrel J G, Mclaughlin S W, Tie L, Laurencin C T, Chen A F, Nair L S (2009). Curcumin-loaded poly(epsilon-caprolactone) nanofibres: Diabetic wound dressing with anti-oxidant and anti-inflammatory properties. Clin. Exp. Pharmacol. Physiol. 36, 1149–1156.
  • Milovanova T, Bhopale V M, Sorokina E M, et al. (2008). Lactate stimulates vasculogenic stem cells via the thioredoxin system and engages an autocrine activation loop involving hypoxia inducible factor-1. Mol Biol Cell. 28:6248.
  • Milovanova T, Bhopale V M, Sorokina E M, et al. (2009). Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo. J Appl Phys.106:711.
  • Öztürk F, Ermertcan A T (2011). Wound healing: a new approach to the topical wound care. Cutan. Ocul. Toxicol. 30,92–99.
  • Panchatcharam M, Miriyala S, Gayathri V S, Suguma L, (2006). Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Mol Cell Biochem. 290(1–2):87–96.
  • Partoazar A, Kianvash N, Darvishi M H, Nasoohi S, Rezayat S M, Bahador A, (2016). Ethosomal curcumin promoted wound healing and reduced bacterial flora in second degree burn in rat. Drug Research. 66(12):660–665.
  • Rai A, Ansul K, Shamimul H, Shazina S, (2019).Curcumin in oral mucosal lesiomns: An Update. Asian J Pharm Clin Res, 12, 2, 32-43.
  • Schierle C F, De La Garza M, Mustoe T A, Galiano R D, (2009) Staphylococcal biofilms impair wound healing by delaying reepithelialization in a murine cutaneous wound model. Wound Rep. Reg. 17:354–359.
  • Schultze-Mosgau S, Wehrhan F, Rodel F. (2003) Improved free vascular graft survival in an irradiated surgical site following topical application of rVEGF. Int J Radiat Oncol Biol Phys; 57:803–12.
  • Sharma A V, Ganguly K, Paul S, Maulik N, Swarnakar S, (2012). Curcumin heals indomethacin-induced gastric ulceration by stimulation of angiogenesis and restitution of collagen fibers via VEGF and mmp-2 mediated signaling. Antioxid Redox Sign. 16(4):351–362.
  • Shen Y, Han C, Chen X, Hou X, Long Z, (2013). Simultaneous determination of three Curcuminoids in Curcuma wenyujin Y.H.chen, C.Ling. By liquid chromatography-tandem mass spectrometry combined with pressurized liquid extraction. J. Pharm. Biomed. 81-82; 146–150.
  • Sugawara J, Akazawa N, Miyaki A, Choi Y, Tanabe Y, Imai T, Maeda S, (2012). Effect of endurance exercise training and curcumin intake oncentral arterial hemodynamics in postmenopausal women: pilot study. Am. J. Hypertens. 25(6):6516.
  • Thangapazham R L, Sharma A, And Maheshwari R K (2007). Beneficial role of curcumin in skin diseases. Adv Exp Med Biol. 595: 343.
  • Velnar T, Bailey T, Smrkol J V (2009). The wound healing process: an 0ver view of the cellular and molecular mechanisms. J.Int. Med. Res. 37,1528–1542.
  • Yoo S Y, Kwon S M (2013) Angiogenesis and Its Therapeutic Opportunities. Mediators of Inflammation. Article ID 127170, p. 11.


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